Properties of the mechanosensitive channel MscS pore revealed by tryptophan scanning mutagenesis

Funding This work was supported by a Wellcome Trust Programme grant [092552/A/10/Z awarded to I.R.B., S.M., J. H. Naismith (University of St Andrews, St Andrews, U.K.), and S. J. Conway (University of Oxford, Oxford, U.K.)] (T.R. and M.D.E.), by a BBSRC grant (A.R.) [BB/H017917/1 awarded to I.R.B., J. H. Naismith, and O. Schiemann (University of St Andrews)], by a Leverhulme Emeritus Fellowship (EM-2012-060\2), and by a CEMI grant to I.R.B. from the California Institute of Technology. The research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007-2013 FP7/2007-2011) under Grant PITN-GA-2011-289384 (FP7-PEOPLE-2011-ITN NICHE) (H.G.) (awarded to S.M.).Peer reviewedPublisher PD

Does cognitive ability influence responses to the Warwick-Edinburgh Mental Well-Being Scale?

It has been suggested that how individuals respond to self-report items relies on cognitive processing. We hypothesized that an individual's level of cognitive ability may influence these processes such that, if there is a hierarchy of items within a particular questionnaire, as demonstrated by Mokken scaling, the strength of that hierarchy will vary according to cognitive ability. Using data on 8,643 men and women from the National Child Development Survey (1958 birth cohort; Power, & Elliott, 2006), we investigated, using Mokken scaling, whether the 14 items that make up the Warwick-Edinburgh Mental Well-Being Scale (Tennant et al., 2007)-completed when the participants were 50 years of age-form a hierarchy and whether that hierarchy varied according to cognitive ability at age 11 years. Among the sample as a whole, we found a moderately strong unidimensional hierarchy of items (Loevinger's coefficient [H] = 0.48). We split participants into 3 groups according to cognitive ability and analyzed the Mokken scaling properties of each group. Only the medium and high cognitive ability groups had acceptable (?0.3) invariant item ordering (assessed using the HT statistic). This pattern was also found when the 3 cognitive ability groups were assessed within men and women separately. Greater attention should be paid to the content validity of questionnaires to ensure they are applicable across the spectrum of mental ability

Overview of Solid Target Studies for a Neutrino Factory

The UK pro­gramme of high power tar­get de­vel­op­ments for a Neu­tri­no Fac­to­ry is cen­tred on the study of high-Z ma­te­ri­als (tung­sten, tan­ta­lum). A de­scrip­tion of life­time shock tests on can­di­date ma­te­ri­als is given as part of the re­search into a solid tar­get so­lu­tion. A fast high cur­rent pulse is ap­plied to a thin wire of the sam­ple ma­te­ri­al and the life­time mea­sured from the num­ber of puls­es be­fore fail­ure. These mea­sure­ments are made at tem­per­a­tures up to ~2000 K. The stress on the wire is cal­cu­lat­ed using the LS-DY­NA code and com­pared to the stress ex­pect­ed in the real Neu­tri­no Fac­to­ry tar­get. It has been found that tan­ta­lum is too weak to sus­tain pro­longed stress at these tem­per­a­tures but a tung­sten wire has reached over 26 mil­lion puls­es (equiv­a­lent to more than ten years of op­er­a­tion at the Neu­tri­no Fac­to­ry). An ac­count is given of the op­ti­mi­sa­tion of sec­ondary pion pro­duc­tion from the tar­get and the is­sues re­lat­ed to mount­ing the tar­get in the muon cap­ture solenoid and tar­get sta­tion are dis­cussed

Coloured mulch as a weed control technology and yield booster for summer savory

An investigation into the effect of coloured mulch technology as a technique to control weeds when growing the essential oil plant, summer savory (Satureja hortensis) was made. As well as weed control, the effects on the production of crop biomass and essential oil content and quality were also considered. The mulch treatments produced significantly more biomass than either of the control treatments (which used no mulch either with or without herbicide). The white mulch treatment produced the greatest biomass, closely followed by the red mulch treatment. The blue mulch treatment was third in ranking, although not significantly greater than the black mulch. Estimates of the quantity of essential oil produced by each treatment followed a similar trend to that shown by biomass production

Randomised positive control trial of NSAID and antimicrobial treatment for calf fever caused by pneumonia

One hundred and fifty-four preweaning calves were followed between May and October 2015. Calves were fitted with continuous monitoring temperature probes (TempVerified FeverTag), programmed so a flashing light emitting diode (LED) light was triggered following six hours of a sustained ear canal temperature of ≥39.7°C. A total of 83 calves (61.9 per cent) developed undifferentiated fever, with a presumptive diagnosis of pneumonia through exclusion of other calf diseases. Once fever was detected, calves were randomly allocated to treatment groups. Calves in group 1 (NSAID) received 2 mg/kg flunixin meglumine (Allevinix, Merial) for three consecutive days and group 2 (antimicrobial) received 6 mg/kg gamithromycin (Zactran, Merial). If fever persisted for 72 hours after the initial treatment, calves were given further treatment (group 1 received antimicrobial and group 2 received NSAID). Calves in group 1 (NSAID) were five times more likely (P=0.002) to require a second treatment (the antimicrobial) after 72 hours to resolve the fever compared with the need to give group 2 (antimicrobial) calves a second treatment (NSAID). This demonstrates the importance of ongoing monitoring and follow-up of calves with respiratory disease. However, of calves with fever in group 1 (NSAID), 25.7 per cent showed resolution following NSAID-only treatment with no detrimental effect on the development of repeated fever or daily live weight gain. This suggests that NSAID alone may be a useful first-line treatment, provided adequate attention is given to ongoing monitoring to identify those cases that require additional antimicrobial treatment

Laser-Doppler gas-velocity instrument

Three-D instrument using a laser light source measures both turbulence and mean velocity of subsonic and supersonic gas flows. This instrument is based on the measurement of the Doppler frequency shift of light waves scattered by moving particles in the gas stream

Horizon energy and angular momentum from a Hamiltonian perspective

Classical black holes and event horizons are highly non-local objects, defined in terms of the causal past of future null infinity. Alternative, (quasi)local definitions are often used in mathematical, quantum, and numerical relativity. These include apparent, trapping, isolated, and dynamical horizons, all of which are closely associated to two-surfaces of zero outward null expansion. In this paper we show that three-surfaces which can be foliated with such two-surfaces are suitable boundaries in both a quasilocal action and a phase space formulation of general relativity. The resulting formalism provides expressions for the quasilocal energy and angular momentum associated with the horizon. The values of the energy and angular momentum are in agreement with those derived from the isolated and dynamical horizon frameworks.Comment: 39 pages, 3 figures, Final Version : content essentially unchanged but many small improvements made in response to referees, a few references adde

Observation of blue-shifted ultralong-range Cs2_{2} Rydberg molecules

We observe ultralong-range blue-shifted Cs$_{2}$ molecular states near $ns_{1/2}$ Rydberg states in an optical dipole trap, where $31\leq n\leq34$. The accidental near degeneracy of $(n-4)l$ and $ns$ Rydberg states for $l>2$ in Cs, due to the small fractional $ns$ quantum defect, leads to non-adiabatic coupling among these states, producing potential wells above the $ns$ thresholds. Two important consequences of admixing high angular momentum states with $ns$ states are the formation of large permanent dipole moments, $\sim 15-100\,$Debye, and accessibility of these states via two-photon association. The observed states are in excellent agreement with theory. Both projections of the total angular momentum on the internuclear axis are visible in the experiment